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Pros and Cons Group 2

Page history last edited by PBworks 17 years, 8 months ago

Pros and Cons of CLM by Rohina Jogi; SEM by Rohina and Grace; TEM by Grace Ngau, revised and edited by Amy Zhao

Table of Pros and Cons

 

Compound Light Microscope
+it has been extremely important in the development of the biological sciences and of medicine
+easy to use
+inexpensive related to electron microscopes
+can look at live samples
-viruses, molecules and atoms cannot be viewed (viewed only with an electron microscope)
-can't magnify more than 2000 times

 

Scanning Electron Microscope
+large depth of field allows more of a specimen to be in focus at one time
+uses electromagnets rather than lenses so the researcher has much more control in the degree of magnification.
+strikingly clear images
-unable to produce colour
-specimen must be electrically conductive

 

Transmission Electron Microscope
+provides information on crystaline structures and density maps
+it enables you to see subatomic resolutions
+requires the sample to be cut into thin slices and placed in a vacuum, therefore there might be damage to the specimen, producing in inaccurate observations
-low contrast
-high resolusion Transmission Electron Microscope is expensive

Sources

 

http://www.labessentials.com/Microscopes_Compound_Basics.htm

 

http://www.madsci.org/posts/archives/jan99/915771165.Gb.r.html

 

http://www.purdue.edu/REM/rs/sem.htm

 

http://www.bwxt.com/operations/semlab.html

 

http://www.nanoword.net/library/defgen/generate.php?termid=109

 

http://www.udel.edu/chem/bahnson/chem645/presentations/Bianco.pdf

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